Hotspot

accD

Genome · 77340-81059 · 3720 bp

Back to Opuntia

Pi 0.0363

MarkerSeek score 74.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3720
variable_sites: 271
pi: 0.0363
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CTCTGGGTCCGGATAAGGAT	GCTCCCATAGAACCCCCTAT	55.0	55.0	58.0	58.0	880-1180 bp	0.833333	83.1
accD_p2	CTCTGGGTCCGGATAAGGAT	CTAGTGGGCGATGCAAGAAT	55.0	50.0	58.0	58.0	1093-1393 bp	0.833333	82.2
accD_p3	CTCTGGGTCCGGATAAGGAT	TTTTCGGTTGTTTGTGCACC	55.0	45.0	58.0	58.0	1234-1534 bp	0.833333	82.0
accD_p4	GGATGAGGATTGTCGAACCC	TTTTCGGTTGTTTGTGCACC	55.0	45.0	58.0	58.0	1377 bp	0.166667	46.3
accD_p5	GGGATGAGGATTGTCGAACC	TTTTCGGTTGTTTGTGCACC	55.0	45.0	58.0	58.0	1378 bp	0.166667	46.3