Hotspot

rpl32-ycf1

Genome · 114612-114669 · 58 bp

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Pi 0.0316

MarkerSeek score 56.60

Primer available yes

Label rpl32-ycf1

Feature Metadata

label_name: rpl32-ycf1
feature_type: igs
region: Genome
length_bp: 58
variable_sites: 4
pi: 0.0316
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf1_p1	GGGATATTGGGCAGCGTTAA	AGCTAGAGATGCGGAATTGC	50.0	50.0	58.0	58.1	301-305 bp	1.0	82.3
rpl32-ycf1_p2	GGGATATTGGGCAGCGTTAA	ACCAGTTGATACGGCTACCT	50.0	50.0	58.0	58.1	463-467 bp	1.0	81.3
rpl32-ycf1_p3	ACGAACAATAGAAGTCGGCC	AGCTAGAGATGCGGAATTGC	50.0	50.0	58.0	58.1	180-185 bp	0.5	63.4
rpl32-ycf1_p4	ACGAACAATAGAAGTCGGCC	ACCAGTTGATACGGCTACCT	50.0	50.0	58.0	58.1	342-347 bp	0.5	61.6
rpl32-ycf1_p5	ACGAACAATAGAAGTCGGCC	GCTACCTCCTTCTCGATTGC	50.0	55.0	58.0	58.1	329-334 bp	0.5	61.5