Hotspot

ndhF-rpl32

Genome · 112921-114341 · 1421 bp

Back to Opuntia

Pi 0.00239

MarkerSeek score 60.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1421
variable_sites: 7
pi: 0.00239
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAACCGGAAGTGGAGTGAAG	TTAACGCTGCCCAATATCCC	55.0	50.0	58.2	58.0	1331-1632 bp	1.0	82.6
ndhF-rpl32_p2	AACAAAGGAACCGGAAGTGG	TTAACGCTGCCCAATATCCC	50.0	50.0	58.3	58.0	1338-1639 bp	1.0	81.7
ndhF-rpl32_p3	TGTTGTCGTCGGAAAAAGGA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.7	58.0	1375-1676 bp	1.0	81.7
ndhF-rpl32_p4	GAACCGGAAGTGGAGTGAAG	AAAAGCTTTTAACGCTGCCC	55.0	45.0	58.2	57.9	1339-1640 bp	1.0	81.6
ndhF-rpl32_p5	AACAAAGGAACCGGAAGTGG	AAAAGCTTTTAACGCTGCCC	50.0	45.0	58.3	57.9	1346-1647 bp	1.0	80.7