Hotspot

psbA-trnH-GUG

Genome · 100840-101211 · 372 bp

Back to Opuntia

Pi 0.00645
MarkerSeek score 55.30
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
Genome
length_bp
372
variable_sites
7
pi
0.00645
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 CTAGACCTAGCCGCTATCGA TTTTGTAGTTGGTGGGCGAA 55.0 45.0 57.9 57.9 466-502 bp 1.0 83.9
psbA-trnH-GUG_p2 TCTAGACCTAGCCGCTATCG TTTTGTAGTTGGTGGGCGAA 55.0 45.0 57.9 57.9 467-503 bp 1.0 83.9
psbA-trnH-GUG_p3 CTAGACCTAGCCGCTATCGA TCACAATCCACTGCCTTGAC 55.0 50.0 57.9 58.1 416-452 bp 1.0 83.7
psbA-trnH-GUG_p4 TCTAGACCTAGCCGCTATCG TCACAATCCACTGCCTTGAC 55.0 50.0 57.9 58.1 417-453 bp 1.0 83.7
psbA-trnH-GUG_p5 CCGTGCTAACCTTGGTATGG TTTTGTAGTTGGTGGGCGAA 55.0 45.0 58.3 57.9 521-557 bp 1.0 81.9