Hotspot

psaJ-trnP-UGG

Genome · 91493-91907 · 415 bp

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Pi 0.0061

MarkerSeek score 54.80

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 415
variable_sites: 7
pi: 0.0061
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	GGTGCCACGGAAAGATATGT	ACCCGTGACATTTTGTACCC	50.0	50.0	58.0	58.1	503-506 bp	1.0	80.3
psaJ-trnP-UGG_p2	GGTGCCACGGAAAGATATGT	CCCGTGACATTTTGTACCCA	50.0	50.0	58.0	58.1	502-505 bp	1.0	80.3
psaJ-trnP-UGG_p3	GGTGCCACGGAAAGATATGT	AAAACAAACGCGCTACCAAG	50.0	45.0	58.0	57.9	483-486 bp	1.0	80.3
psaJ-trnP-UGG_p4	GGTGCCACGGAAAGATATGT	GGGATTTGAACCCGTGACAT	50.0	50.0	58.0	58.2	512-515 bp	1.0	79.8
psaJ-trnP-UGG_p5	GGTGCCACGGAAAGATATGT	CAAAACAAACGCGCTACCAA	50.0	45.0	58.0	58.2	484-487 bp	1.0	79.8