Hotspot

ndhC-rbcL

Genome · 70732-71529 · 798 bp

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Pi 0.00359

MarkerSeek score 54.40

Primer available yes

Label ndhC-rbcL

Feature Metadata

label_name: ndhC-rbcL
feature_type: igs
region: Genome
length_bp: 798
variable_sites: 7
pi: 0.00359
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-rbcL_p1	GTCCTTTGCTAATCGGAGCT	AAATTTGAGTTCCCGGCAGT	50.0	45.0	57.7	58.0	939-961 bp	1.0	79.6
ndhC-rbcL_p2	GTCCTTTGCTAATCGGAGCT	GCTGCTTGTGAGGTATGGAA	50.0	50.0	57.7	57.9	966-988 bp	1.0	78.7
ndhC-rbcL_p3	GTCCTTTGCTAATCGGAGCT	CGGCAGTGGATACTTTGGAT	50.0	50.0	57.7	57.7	926-948 bp	1.0	77.0
ndhC-rbcL_p4	CTGGTCCTTTGCTAATCGGA	AAATTTGAGTTCCCGGCAGT	50.0	45.0	57.3	58.0	942-964 bp	1.0	76.6
ndhC-rbcL_p5	TGGTCCTTTGCTAATCGGAG	AAATTTGAGTTCCCGGCAGT	50.0	45.0	57.3	58.0	941-963 bp	1.0	76.6