Hotspot

psaA-ycf3

Genome · 60947-61767 · 821 bp

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Pi 0.00325

MarkerSeek score 54.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 821
variable_sites: 6
pi: 0.00325
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	CGATAACGCTTACTCCCGAG	50.0	55.0	58.1	58.0	950-982 bp	1.0	81.9
psaA-ycf3_p2	GTTCTTGAAAAATGGCCCGG	CGATAACGCTTACTCCCGAG	50.0	55.0	57.9	58.0	969-1001 bp	1.0	81.8
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	977-1009 bp	1.0	81.7
psaA-ycf3_p4	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	996-1028 bp	1.0	81.7
psaA-ycf3_p5	GTCTGGCCCATTCCTCAAAA	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	979-1011 bp	1.0	80.9