primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ycf2	1	AATCATGGTCCTTGCGGATC	CTTTGACCCCCGAATTTGGA	20	20	50.0	50.0	58.0	58.1	0.0	13.8	0.109256	11976	12095	512-512	0.500000	59.9
ycf2_p2	ycf2	2	AATCATGGTCCTTGCGGATC	TCTTTGACCCCCGAATTTGG	20	20	50.0	50.0	58.0	58.1	0.0	4.5	0.109256	11976	12095	513-513	0.500000	59.9
ycf2_p3	ycf2	3	AATCATGGTCCTTGCGGATC	TTTGACCCCCGAATTTGGAG	20	20	50.0	50.0	58.0	58.1	0.0	13.8	0.109256	11976	12095	511-511	0.500000	59.9
ycf2_p4	ycf2	4	GTACGGATTCTTCCCCCAAC	CTTTGACCCCCGAATTTGGA	20	20	55.0	50.0	58.0	58.1	0.0	13.8	0.116232	11976	12095	817-817	0.500000	59.8
ycf2_p5	ycf2	5	GTACGGATTCTTCCCCCAAC	TTTGACCCCCGAATTTGGAG	20	20	55.0	50.0	58.0	58.1	0.0	13.8	0.116232	11976	12095	816-816	0.500000	59.8
petD_p1	petD	1	ACTTTTGAAGCGACTATTCCCT	TGGACATTTTCCTTCAACTCCA	22	22	40.9	40.9	57.7	57.8	0.0	0.0	4.465303	20591	21860	1422-1428	1.000000	45.0
petD_p2	petD	2	CACTTTTGAAGCGACTATTCCC	TGGACATTTTCCTTCAACTCCA	22	22	45.5	40.9	57.7	57.8	0.0	0.0	4.519889	20591	21860	1423-1429	1.000000	44.5
petD_p3	petD	3	ACTTTTGAAGCGACTATTCCCT	GGACATTTTCCTTCAACTCCAC	22	22	40.9	45.5	57.7	57.5	0.0	0.0	4.793079	20591	21860	1421-1427	1.000000	42.3
petD_p4	petD	4	CACTTTTGAAGCGACTATTCCC	GGACATTTTCCTTCAACTCCAC	22	22	45.5	45.5	57.7	57.5	0.0	0.0	4.847664	20591	21860	1422-1428	1.000000	41.9
petD_p5	petD	5	TCCGAATAGGCCCATAATTTCA	TGGACATTTTCCTTCAACTCCA	22	22	40.9	40.9	57.3	57.8	0.0	0.0	4.893072	20591	21860	1476-1482	1.000000	41.5
psaA-ycf3_p1	psaA-ycf3	1	GTCTGGCCCATTCCTCAAAA	CGATAACGCTTACTCCCGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.110437	60947	61767	950-982	1.000000	81.9
psaA-ycf3_p2	psaA-ycf3	2	GTTCTTGAAAAATGGCCCGG	CGATAACGCTTACTCCCGAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.112642	60947	61767	969-1001	1.000000	81.8
psaA-ycf3_p3	psaA-ycf3	3	GTCTGGCCCATTCCTCAAAA	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117603	60947	61767	977-1009	1.000000	81.7
psaA-ycf3_p4	psaA-ycf3	4	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119807	60947	61767	996-1028	1.000000	81.7
psaA-ycf3_p5	psaA-ycf3	5	GTCTGGCCCATTCCTCAAAA	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.218553	60947	61767	979-1011	1.000000	80.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	68228	69013	928-978	1.000000	82.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	68228	69013	892-942	1.000000	82.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	TAATCACCCACGCCTGAAAC	20	20	55.0	50.0	58.0	58.2	10.3	0.0	0.186431	68228	69013	965-1015	1.000000	81.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	68228	69013	916-966	1.000000	81.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TAATCACCCACGCCTGAAAC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.235821	68228	69013	929-979	1.000000	81.4
ndhC-rbcL_p1	ndhC-rbcL	1	GTCCTTTGCTAATCGGAGCT	AAATTTGAGTTCCCGGCAGT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.322153	70732	71529	939-961	1.000000	79.6
ndhC-rbcL_p2	ndhC-rbcL	2	GTCCTTTGCTAATCGGAGCT	GCTGCTTGTGAGGTATGGAA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.431390	70732	71529	966-988	1.000000	78.7
ndhC-rbcL_p3	ndhC-rbcL	3	GTCCTTTGCTAATCGGAGCT	CGGCAGTGGATACTTTGGAT	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.649593	70732	71529	926-948	1.000000	77.0
ndhC-rbcL_p4	ndhC-rbcL	4	CTGGTCCTTTGCTAATCGGA	AAATTTGAGTTCCCGGCAGT	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.696032	70732	71529	942-964	1.000000	76.6
ndhC-rbcL_p5	ndhC-rbcL	5	TGGTCCTTTGCTAATCGGAG	AAATTTGAGTTCCCGGCAGT	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.696032	70732	71529	941-963	1.000000	76.6
accD_p1	accD	1	CTCTGGGTCCGGATAAGGAT	GCTCCCATAGAACCCCCTAT	20	20	55.0	55.0	58.0	58.0	7.8	0.0	0.063185	80077	80196	880-1180	0.833333	83.1
accD_p2	accD	2	CTCTGGGTCCGGATAAGGAT	CTAGTGGGCGATGCAAGAAT	20	20	55.0	50.0	58.0	58.0	7.8	0.0	0.057863	80077	80196	1093-1393	0.833333	82.2
accD_p3	accD	3	CTCTGGGTCCGGATAAGGAT	TTTTCGGTTGTTTGTGCACC	20	20	55.0	45.0	58.0	58.0	7.8	15.7	0.019236	80077	80196	1234-1534	0.833333	82.0
accD_p4	accD	4	GGATGAGGATTGTCGAACCC	TTTTCGGTTGTTTGTGCACC	20	20	55.0	45.0	58.0	58.0	0.0	15.7	0.047404	80077	80196	1377-1377	0.166667	46.3
accD_p5	accD	5	GGGATGAGGATTGTCGAACC	TTTTCGGTTGTTTGTGCACC	20	20	55.0	45.0	58.0	58.0	0.0	15.7	0.047404	80077	80196	1378-1378	0.166667	46.3
psaJ-trnP-UGG_p1	psaJ-trnP-UGG	1	GGTGCCACGGAAAGATATGT	ACCCGTGACATTTTGTACCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.138141	91493	91907	503-506	1.000000	80.3
psaJ-trnP-UGG_p2	psaJ-trnP-UGG	2	GGTGCCACGGAAAGATATGT	CCCGTGACATTTTGTACCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.139485	91493	91907	502-505	1.000000	80.3
psaJ-trnP-UGG_p3	psaJ-trnP-UGG	3	GGTGCCACGGAAAGATATGT	AAAACAAACGCGCTACCAAG	20	20	50.0	45.0	58.0	57.9	0.0	5.7	0.152297	91493	91907	483-486	1.000000	80.3
psaJ-trnP-UGG_p4	psaJ-trnP-UGG	4	GGTGCCACGGAAAGATATGT	GGGATTTGAACCCGTGACAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.204199	91493	91907	512-515	1.000000	79.8
psaJ-trnP-UGG_p5	psaJ-trnP-UGG	5	GGTGCCACGGAAAGATATGT	CAAAACAAACGCGCTACCAA	20	20	50.0	45.0	58.0	58.2	0.0	5.7	0.209555	91493	91907	484-487	1.000000	79.8
psbA-trnH-GUG_p1	psbA-trnH-GUG	1	CTAGACCTAGCCGCTATCGA	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.150452	100840	101211	466-502	1.000000	83.9
psbA-trnH-GUG_p2	psbA-trnH-GUG	2	TCTAGACCTAGCCGCTATCG	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.150452	100840	101211	467-503	1.000000	83.9
psbA-trnH-GUG_p3	psbA-trnH-GUG	3	CTAGACCTAGCCGCTATCGA	TCACAATCCACTGCCTTGAC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.200000	100840	101211	416-452	1.000000	83.7
psbA-trnH-GUG_p4	psbA-trnH-GUG	4	TCTAGACCTAGCCGCTATCG	TCACAATCCACTGCCTTGAC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.200000	100840	101211	417-453	1.000000	83.7
psbA-trnH-GUG_p5	psbA-trnH-GUG	5	CCGTGCTAACCTTGGTATGG	TTTTGTAGTTGGTGGGCGAA	20	20	55.0	45.0	58.3	57.9	0.0	0.0	0.390538	100840	101211	521-557	1.000000	81.9
ndhF-rpl32_p1	ndhF-rpl32	1	GAACCGGAAGTGGAGTGAAG	TTAACGCTGCCCAATATCCC	20	20	55.0	50.0	58.2	58.0	9.5	0.0	0.227316	112921	114341	1331-1632	1.000000	82.6
ndhF-rpl32_p2	ndhF-rpl32	2	AACAAAGGAACCGGAAGTGG	TTAACGCTGCCCAATATCCC	20	20	50.0	50.0	58.3	58.0	9.5	0.0	0.336778	112921	114341	1338-1639	1.000000	81.7
ndhF-rpl32_p3	ndhF-rpl32	3	TGTTGTCGTCGGAAAAAGGA	TTAACGCTGCCCAATATCCC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.337063	112921	114341	1375-1676	1.000000	81.7
ndhF-rpl32_p4	ndhF-rpl32	4	GAACCGGAAGTGGAGTGAAG	AAAAGCTTTTAACGCTGCCC	20	20	55.0	45.0	58.2	57.9	9.5	22.7	0.351891	112921	114341	1339-1640	1.000000	81.6
ndhF-rpl32_p5	ndhF-rpl32	5	AACAAAGGAACCGGAAGTGG	AAAAGCTTTTAACGCTGCCC	20	20	50.0	45.0	58.3	57.9	9.5	22.7	0.461353	112921	114341	1346-1647	1.000000	80.7
rpl32-ycf1_p1	rpl32-ycf1	1	GGGATATTGGGCAGCGTTAA	AGCTAGAGATGCGGAATTGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.148018	114612	114669	301-305	1.000000	82.3
rpl32-ycf1_p2	rpl32-ycf1	2	GGGATATTGGGCAGCGTTAA	ACCAGTTGATACGGCTACCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.162358	114612	114669	463-467	1.000000	81.3
rpl32-ycf1_p3	rpl32-ycf1	3	ACGAACAATAGAAGTCGGCC	AGCTAGAGATGCGGAATTGC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.129649	114612	114669	180-185	0.500000	63.4
rpl32-ycf1_p4	rpl32-ycf1	4	ACGAACAATAGAAGTCGGCC	ACCAGTTGATACGGCTACCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.143989	114612	114669	342-347	0.500000	61.6
rpl32-ycf1_p5	rpl32-ycf1	5	ACGAACAATAGAAGTCGGCC	GCTACCTCCTTCTCGATTGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.146588	114612	114669	329-334	0.500000	61.5
ycf1_p1	ycf1	1	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTATAGT	20	23	50.0	39.1	57.9	56.3	15.9	26.3	4.824564	133089	135941	2164-3000	0.333333	19.4
ycf1_p2	ycf1	2	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTAT	20	20	50.0	40.0	57.9	53.2	15.9	26.3	4.881243	133089	135941	2164-3000	0.333333	19.0
ycf1_p3	ycf1	3	GCGTGCGCATCTATTTATCT	TGGAACGATGATGCATCTTA	20	20	45.0	40.0	56.5	54.6	15.9	26.3	4.969269	133089	135941	2164-3000	0.333333	18.3
ycf1_p4	ycf1	4	GCGTGCGCATCTATTTATCT	TGGAACGATGATGCATCTTAT	20	21	45.0	38.1	56.5	55.2	15.9	26.3	5.354960	133089	135941	2164-3000	0.333333	18.0
ycf1_p5	ycf1	5	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTATAG	20	22	50.0	40.9	57.9	54.6	15.9	26.3	5.514875	133089	135941	2164-3000	0.333333	18.0
ycf1-rpl32_p1	ycf1-rpl32	1	AGCTAGAGATGCGGAATTGC	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148018	135942	135999	301-305	1.000000	82.3
ycf1-rpl32_p2	ycf1-rpl32	2	ACCAGTTGATACGGCTACCT	GGGATATTGGGCAGCGTTAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.162358	135942	135999	463-467	1.000000	81.3
ycf1-rpl32_p3	ycf1-rpl32	3	AGCTAGAGATGCGGAATTGC	ACGAACAATAGAAGTCGGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.129649	135942	135999	180-185	0.500000	63.8
ycf1-rpl32_p4	ycf1-rpl32	4	ACCAGTTGATACGGCTACCT	ACGAACAATAGAAGTCGGCC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.143989	135942	135999	342-347	0.500000	61.9
ycf1-rpl32_p5	ycf1-rpl32	5	GCTACCTCCTTCTCGATTGC	ACGAACAATAGAAGTCGGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.146588	135942	135999	329-334	0.500000	61.7
rpl32-ndhF_p1	rpl32-ndhF	1	TTAACGCTGCCCAATATCCC	GAACCGGAAGTGGAGTGAAG	20	20	50.0	55.0	58.0	58.2	0.0	9.5	0.227316	136270	137690	1331-1632	1.000000	82.6
rpl32-ndhF_p2	rpl32-ndhF	2	TTAACGCTGCCCAATATCCC	AACAAAGGAACCGGAAGTGG	20	20	50.0	50.0	58.0	58.3	0.0	9.5	0.336778	136270	137690	1338-1639	1.000000	81.7
rpl32-ndhF_p3	rpl32-ndhF	3	TTAACGCTGCCCAATATCCC	TGTTGTCGTCGGAAAAAGGA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.337063	136270	137690	1375-1676	1.000000	81.7
rpl32-ndhF_p4	rpl32-ndhF	4	AAAAGCTTTTAACGCTGCCC	GAACCGGAAGTGGAGTGAAG	20	20	45.0	55.0	57.9	58.2	22.7	9.5	0.351891	136270	137690	1339-1640	1.000000	81.6
rpl32-ndhF_p5	rpl32-ndhF	5	AAAAGCTTTTAACGCTGCCC	AACAAAGGAACCGGAAGTGG	20	20	45.0	50.0	57.9	58.3	22.7	9.5	0.461353	136270	137690	1346-1647	1.000000	80.7