Hotspot

rps4

LSC · 47775-48380 · 606 bp

Back to Oplopanax

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AAGGGAGCAAAATTGTCCGA	ACTTCCGTAAACTTCGCTTCA	45.0	42.9	57.7	57.9	773 bp	1.0	69.0
rps4_p2	AGGGAGCAAAATTGTCCGAA	ACTTCCGTAAACTTCGCTTCA	45.0	42.9	57.7	57.9	772 bp	1.0	69.0
rps4_p3	ACTTCAACGAAAGGGAGCAA	ACTTCCGTAAACTTCGCTTCA	45.0	42.9	57.7	57.9	783 bp	1.0	68.6
rps4_p4	GGAGCAAAATTGTCCGAACC	ACTTCCGTAAACTTCGCTTCA	50.0	42.9	57.7	57.9	770 bp	1.0	68.5
rps4_p5	GGGAGCAAAATTGTCCGAAC	ACTTCCGTAAACTTCGCTTCA	50.0	42.9	57.7	57.9	771 bp	1.0	68.5