Hotspot

rps2

LSC · 16798-17508 · 711 bp

Back to Oplopanax

Pi 0
MarkerSeek score 27.00
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 GCGCTCCTGACCAATTAGAA AATGGCTTGGGTCGTGTATC 50.0 50.0 58.0 58.0 1419-1421 bp 1.0 79.6
rps2_p2 AAGCGCTCCTGACCAATTAG AATGGCTTGGGTCGTGTATC 50.0 50.0 58.0 58.0 1421-1423 bp 1.0 79.6
rps2_p3 ACGTGAGTAAAGCCAAAGCA AATGGCTTGGGTCGTGTATC 45.0 50.0 58.0 58.0 1512-1514 bp 1.0 79.5
rps2_p4 CTTCCACACCGGCTATATCG AATGGCTTGGGTCGTGTATC 55.0 50.0 57.9 58.0 1143-1145 bp 1.0 79.3
rps2_p5 GCGCTCCTGACCAATTAGAA AGGCCTGAAAGAGAACGTTG 50.0 50.0 58.0 58.1 1746-1748 bp 1.0 79.0