Hotspot

matK

LSC · 2066-3583 · 1518 bp

Back to Oplopanax

Pi 0.00132
MarkerSeek score 59.50
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1518
variable_sites
2
pi
0.00132
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 TTACACGGATTCCTAACGAA CCTTGTTTTGACTGTATCGCA 40.0 42.9 54.1 56.8 1617 bp 1.0 40.1
matK_p2 TTTACACGGATTCCTAACGA CCTTGTTTTGACTGTATCGCA 40.0 42.9 54.1 56.8 1618 bp 1.0 40.1
matK_p3 TTACACGGATTCCTAACGAA ACCTTGTTTTGACTGTATCGC 40.0 42.9 54.1 56.8 1618 bp 1.0 40.1
matK_p4 TTTACACGGATTCCTAACGA ACCTTGTTTTGACTGTATCGC 40.0 42.9 54.1 56.8 1619 bp 1.0 40.1
matK_p5 TTACACGGATTCCTAACGAA CCTTGTTTTGACTGTATCGCAC 40.0 45.5 54.1 58.2 1617 bp 1.0 40.1