Hotspot

ccsA

SSC · 116569-117537 · 969 bp

Back to Oplopanax

Pi 0.00206

MarkerSeek score 59.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCAGACCTTCTTGTAAA	ATTCACATGGTTCTCGTCGG	50.0	50.0	58.5	58.0	1129 bp	1.0	76.2
ccsA_p2	GCGGCAGACCTTCTTGTAAA	GGTCTTTGTGAGAACCGTGT	50.0	50.0	58.5	58.1	1167 bp	1.0	75.9
ccsA_p3	GCGGCAGACCTTCTTGTAAA	TGGTCTTTGTGAGAACCGTG	50.0	50.0	58.5	58.1	1168 bp	1.0	75.9
ccsA_p4	GCGGCAGACCTTCTTGTAAA	CATGGTTCTCGTCGGTTCTT	50.0	50.0	58.5	57.9	1124 bp	1.0	75.7
ccsA_p5	GCGGCAGACCTTCTTGTAAA	TGTGAGAACCGTGTGAATCC	50.0	50.0	58.5	57.8	1161 bp	1.0	75.0