Hotspot

atpF

LSC · 12794-14072 · 1279 bp

Back to Oplopanax

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1279
variable_sites: 2
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AAAGAACGGGGGCAAAGTTA	50.0	45.0	57.7	57.6	1437 bp	1.0	74.6
atpF_p2	GGCTCGAATGGTTACCATGA	AAAAAGAACGGGGGCAAAGT	50.0	45.0	57.7	58.5	1439 bp	1.0	73.4
atpF_p3	GGCTCGAATGGTTACCATGA	AAAAGAACGGGGGCAAAGTT	50.0	45.0	57.7	58.5	1438 bp	1.0	73.4
atpF_p4	GGCTCGAATGGTTACCATGA	AAGAACGGGGGCAAAGTTAT	50.0	45.0	57.7	57.4	1436 bp	1.0	72.7
atpF_p5	ACTAATTTCGTCGGCTCGAA	AAAGAACGGGGGCAAAGTTA	45.0	45.0	57.4	57.6	1449 bp	1.0	72.0