Hotspot

atpB-rbcL

LSC · 56507-57266 · 760 bp

Back to Oplopanax

Pi 0.00132

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 1
pi: 0.00132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGTGATATTCCCCAGGTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	904 bp	1.0	71.9
atpB-rbcL_p2	TGGGTGATATTCCCCAGGTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	903 bp	1.0	71.6
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	927 bp	1.0	71.6
atpB-rbcL_p4	TGGGTGATATTCCCCAGGTT	GTGGTGACATAAATCCCTCCC	50.0	52.4	58.0	58.1	865 bp	1.0	71.5
atpB-rbcL_p5	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	926 bp	1.0	71.3