Hotspot

psbZ-trnG-UCC

LSC · 38160-38445 · 286 bp

Back to Oplopanax

Pi 0

MarkerSeek score 53.50

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 286
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CCTTCTCCTTGGCAAAGAGA	50.0	50.0	57.8	57.1	438-441 bp	1.0	72.0
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CCTTCTCCTTGGCAAAGAGA	55.0	50.0	58.3	57.1	436-439 bp	1.0	71.6
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	CCTTCTCCTTGGCAAAGAGAA	50.0	47.6	57.8	57.9	438-441 bp	1.0	69.7
psbZ-trnG-UCC_p4	TTGCTTCTCCTGATGGTTGG	AAATTGCTCCATCTTGGGCG	50.0	50.0	57.8	59.2	480-483 bp	1.0	69.4
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	462-465 bp	1.0	69.4