Hotspot

trnE-UUC-trnT-GGU

LSC · 32648-33450 · 803 bp

Back to Oplopanax

Pi 0.00126

MarkerSeek score 60.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	937-944 bp	1.0	79.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	900-907 bp	1.0	79.3
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.8	57.9	902-909 bp	1.0	78.6
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	865-872 bp	1.0	78.5
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	878-885 bp	1.0	78.4