Hotspot

petN-psbM

LSC · 30209-30982 · 774 bp

Back to Oplopanax

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	932-937 bp	1.0	68.8
petN-psbM_p2	ACATTTTCCCTTTCGCTCGT	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.1	57.6	901-906 bp	1.0	68.0
petN-psbM_p3	CCCTTTCGCTCGTAGTATGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	894-899 bp	1.0	67.7
petN-psbM_p4	CCTTTCGCTCGTAGTATGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	893-898 bp	1.0	67.7
petN-psbM_p5	CTTTCGCTCGTAGTATGGGG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	892-897 bp	1.0	67.7