Pi
0.00299
MarkerSeek score
39.40
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | AGAAAAGAAAAAGGGAGGTTTGTG | TTAAATCGGTAGGGGGTGGG | 37.5 | 55.0 | 58.0 | 58.8 | 724-733 bp | 1.0 | 42.6 |
| ycf1_p2 | AAGAAAAAGGGAGGTTTGTG | TTAAATCGGTAGGGGGTGGG | 40.0 | 55.0 | 53.9 | 58.8 | 720-729 bp | 1.0 | 41.8 |
| ycf1_p3 | AGAAAAGAAAAAGGGAGGTTTGTG | GGTGGGCGTATTTCCTCTTA | 37.5 | 50.0 | 58.0 | 57.0 | 710-719 bp | 1.0 | 41.1 |
| ycf1_p4 | AAAGAAAAAGGGAGGTTTGTG | TTAAATCGGTAGGGGGTGGG | 38.1 | 55.0 | 54.8 | 58.8 | 721-730 bp | 1.0 | 41.1 |
| ycf1_p5 | AGAAAAGAAAAAGGGAGGTTTGT | TTAAATCGGTAGGGGGTGGG | 34.8 | 55.0 | 56.7 | 58.8 | 724-733 bp | 1.0 | 41.1 |
| ycf1_p1 | AGTTTTTCGATCGATTCTAAAA | TGGTTTATCCCAAGCAACTT | 27.3 | 40.0 | 52.2 | 55.1 | 194 bp | 1.0 | 40.8 |
| ycf1_p2 | AGTTTTTCGATCGATTCTAAAA | TTGGTTTATCCCAAGCAACT | 27.3 | 40.0 | 52.2 | 55.1 | 195 bp | 1.0 | 40.8 |
| ycf1_p3 | TTTTAGTTTTTCGATCGATTCT | TGGTTTATCCCAAGCAACTT | 27.3 | 40.0 | 52.2 | 55.1 | 198 bp | 1.0 | 40.8 |
| ycf1_p4 | TTTTAGTTTTTCGATCGATTCT | TTGGTTTATCCCAAGCAACT | 27.3 | 40.0 | 52.2 | 55.1 | 199 bp | 1.0 | 40.8 |
| ycf1_p5 | AGTTTTTCGATCGATTCTAAAA | AGAGAAACCTTGGTTTATCCCA | 27.3 | 40.9 | 52.2 | 57.0 | 204 bp | 1.0 | 40.8 |