Hotspot

rpoA

LSC · 76640-77647 · 1008 bp

Back to Opisthopappus

Pi 0.00397

MarkerSeek score 61.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 4
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATCTATGCCGATTCCAA	CATAATGGCTGTAGACCCCC	40.0	55.0	54.5	57.4	1223 bp	1.0	47.8
rpoA_p2	TGAATCTATGCCGATTCCAAA	CATAATGGCTGTAGACCCCC	38.1	55.0	55.4	57.4	1223 bp	1.0	46.6
rpoA_p3	ATGAATCTATGCCGATTCCA	CATAATGGCTGTAGACCCCC	40.0	55.0	54.3	57.4	1224 bp	1.0	45.9
rpoA_p4	TGAATCTATGCCGATTCCAA	ATAATGGCTGTAGACCCCCG	40.0	55.0	54.5	58.9	1222 bp	1.0	44.8
rpoA_p5	ATGAATCTATGCCGATTCCAA	CATAATGGCTGTAGACCCCC	38.1	55.0	55.1	57.4	1224 bp	1.0	44.8