Hotspot

psaC

SSC · 115149-115394 · 246 bp

Back to Opisthopappus

Pi 0
MarkerSeek score 23.50
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 GCACATGTTTTGGCGGTAAA TCAATCCAGAAAAACCGGGT 45.0 45.0 57.9 57.3 416 bp 1.0 73.5
psaC_p2 TTTTGGGCACATGTTTTGGC TCAATCCAGAAAAACCGGGT 45.0 45.0 58.3 57.3 422 bp 1.0 72.0
psaC_p3 GCACATGTTTTGGCGGTAAA CCGGGTAACTCATCGATTCA 45.0 50.0 57.9 57.1 402 bp 1.0 71.8
psaC_p4 GCACATGTTTTGGCGGTAAA ACCGGGTAACTCATCGATTC 45.0 50.0 57.9 57.1 403 bp 1.0 71.8
psaC_p5 GCACATGTTTTGGCGGTAAA GTCAATCCAGAAAAACCGGG 45.0 50.0 57.9 57.0 417 bp 1.0 70.8