Hotspot

petD

LSC · 75273-76430 · 1158 bp

Back to Opisthopappus

Pi 0.00259

MarkerSeek score 60.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1158
variable_sites: 3
pi: 0.00259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGCATCAATAGTTGAAGGGA	ACTTTGAAGTGACTGCTCCC	42.9	50.0	57.0	57.7	1265-1266 bp	1.0	61.9
petD_p2	TGGCATCAATAGTTGAAGGGA	CTTTGAAGTGACTGCTCCCT	42.9	50.0	57.0	57.4	1264-1265 bp	1.0	59.6
petD_p3	TGGCATCAATAGTTGAAGGGAA	ACTTTGAAGTGACTGCTCCC	40.9	50.0	57.7	57.7	1265-1266 bp	1.0	59.6
petD_p4	TTGGCATCAATAGTTGAAGGGA	ACTTTGAAGTGACTGCTCCC	40.9	50.0	57.7	57.7	1266-1267 bp	1.0	59.6
petD_p5	TGGCATCAATAGTTGAAGGGA	TGAAGTGACTGCTCCCTAGA	42.9	50.0	57.0	57.4	1261-1262 bp	1.0	59.2