Hotspot

ndhG-ndhI

SSC · 116665-117022 · 358 bp

Back to Opisthopappus

Pi 0.00559

MarkerSeek score 55.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 358
variable_sites: 2
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TACTACTCCCAGACCCCCTA	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.7	58.3	527 bp	1.0	75.4
ndhG-ndhI_p2	ATACTACTCCCAGACCCCCT	TCAAATTGCTTTGGGTCGGT	55.0	45.0	58.4	58.3	528 bp	1.0	75.0
ndhG-ndhI_p3	AATACTACTCCCAGACCCCC	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.5	58.3	529 bp	1.0	74.0
ndhG-ndhI_p4	TACTACTCCCAGACCCCCTA	CAAATTGCTTTGGGTCGGTT	55.0	45.0	57.7	57.5	526 bp	1.0	73.6
ndhG-ndhI_p5	ATACTACTCCCAGACCCCCT	CAAATTGCTTTGGGTCGGTT	55.0	45.0	58.4	57.5	527 bp	1.0	73.2