Hotspot

ndhD-psaC

SSC · 115036-115148 · 113 bp

Back to Opisthopappus

Pi 0.0265

MarkerSeek score 67.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 3
pi: 0.0265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AGGAACCCGCAAATATTGGT	CGGCTGTAAGAGATGCGAAT	45.0	50.0	57.5	58.1	271 bp	1.0	75.5
ndhD-psaC_p2	AGGAACCCGCAAATATTGGT	GAGGATTGTGTCGGCTGTAA	45.0	50.0	57.5	57.6	282 bp	1.0	73.6
ndhD-psaC_p3	AGGAACCCGCAAATATTGGT	GGATTGTGTCGGCTGTAAGA	45.0	50.0	57.5	57.6	280 bp	1.0	73.6
ndhD-psaC_p4	AGGAACCCGCAAATATTGGT	AGGATTGTGTCGGCTGTAAG	45.0	50.0	57.5	57.6	281 bp	1.0	73.6
ndhD-psaC_p5	AGGAACCCGCAAATATTGGT	AGAGGATTGTGTCGGCTGTA	45.0	50.0	57.5	58.4	283 bp	1.0	73.1