Hotspot

psaA-ycf3

LSC · 41199-41934 · 736 bp

Back to Opisthopappus

Pi 0.00138

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 1
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	55.0	50.0	57.8	58.3	919-928 bp	1.0	77.1
psaA-ycf3_p2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	55.0	50.0	57.8	58.3	921-930 bp	1.0	77.0
psaA-ycf3_p3	GGGCCTTTCGCTATTGTTCT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.2	58.3	940-949 bp	1.0	76.9
psaA-ycf3_p4	GGGCCTTTCGCTATTGTTCT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.2	58.3	942-951 bp	1.0	76.8
psaA-ycf3_p5	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	55.0	45.0	57.8	57.5	920-929 bp	1.0	75.2