Hotspot

trnK-UUU-rps16

LSC · 4415-5262 · 848 bp

Back to Opisthopappus

Pi 0.00355

MarkerSeek score 61.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 3
pi: 0.00355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	1002-1004 bp	1.0	78.2
trnK-UUU-rps16_p2	CGATGGTATGGACGAATCCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	1001-1003 bp	1.0	78.2
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	941-943 bp	1.0	77.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	940-942 bp	1.0	77.6
trnK-UUU-rps16_p5	TCAGTCGTGGTCTTCCAAAC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.8	58.2	1026-1028 bp	1.0	77.0