Hotspot

ycf1

SSC · 116141-121459 · 5319 bp

Pi 0.0127

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5319
variable_sites: 100
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTGGGACAGGGAAGATA	ACAGTCTGGGGAACGAAAAC	50.0	50.0	58.0	58.0	819-843 bp	1.0	81.7
ycf1_p2	TATGCTTGGGACAGGGAAGA	ACAGTCTGGGGAACGAAAAC	50.0	50.0	58.0	58.0	821-845 bp	1.0	81.6
ycf1_p3	TATGCTTGGGACAGGGAAGA	CAGTCTGGGGAACGAAAACA	50.0	50.0	58.0	58.0	820-844 bp	1.0	81.6
ycf1_p4	TGCTTGGGACAGGGAAGATA	CAGTCTGGGGAACGAAAACA	50.0	50.0	58.0	58.0	818-842 bp	1.0	81.6
ycf1_p5	TGCTTGGGACAGGGAAGATA	ATTCCCTTCGGTTCTCTCCA	50.0	50.0	58.0	58.0	795 bp	0.666667	67.0