Hotspot

ccsA-ndhD

SSC · 107930-109235 · 1306 bp

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Pi 0.0158
MarkerSeek score 54.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
1306
variable_sites
31
pi
0.0158
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGCTACGTCAGGTCTTTCAA GCTTGTCCCACGGATGTATT 50.0 50.0 57.9 58.0 2693-2738 bp 1.0 80.6
ccsA-ndhD_p2 GTTTCGCTACGTCAGGTCTT GCTTGTCCCACGGATGTATT 50.0 50.0 57.9 58.0 2697-2742 bp 1.0 80.6
ccsA-ndhD_p3 CAAGGCACAAATTCGGCATT GCTTGTCCCACGGATGTATT 45.0 50.0 57.9 58.0 2176-2221 bp 1.0 80.5
ccsA-ndhD_p4 GATCAAGGCACAAATTCGGC GCTTGTCCCACGGATGTATT 50.0 50.0 58.1 58.0 2179-2224 bp 1.0 80.5
ccsA-ndhD_p5 TTACCCAAGGTTTCGCTACG GCTTGTCCCACGGATGTATT 50.0 50.0 57.9 58.0 2706-2751 bp 1.0 80.4