Hotspot

rps8-rpl14

LSC · 76941-77168 · 228 bp

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Pi 0.0175
MarkerSeek score 66.00
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
228
variable_sites
5
pi
0.0175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 CGGCAATAATGTCCCTACCC CGATTGCTCGGGAATTGAGA 55.0 50.0 58.1 58.1 309-318 bp 1.0 83.0
rps8-rpl14_p2 CGGCAATAATGTCCCTACCC GAACTCGGATTTTTGGTGCG 55.0 50.0 58.1 58.0 327-336 bp 1.0 82.9
rps8-rpl14_p3 CGGCAATAATGTCCCTACCC TTGCTCGGGAATTGAGACAG 55.0 50.0 58.1 57.9 306-315 bp 1.0 82.7
rps8-rpl14_p4 CGGCAATAATGTCCCTACCC GCTCGGGAATTGAGACAGTT 55.0 50.0 58.1 57.9 304-313 bp 1.0 82.7
rps8-rpl14_p5 CGGCAATAATGTCCCTACCC ACTCGGATTTTTGGTGCGAT 55.0 45.0 58.1 58.2 325-334 bp 1.0 81.8