Hotspot

infA-rps8

LSC · 76431-76544 · 114 bp

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Pi 0.0175
MarkerSeek score 63.70
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
114
variable_sites
3
pi
0.0175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 ACCGTTGGGAAGTGATTCAG GGCGGAATGGGGATTGTAAT 50.0 50.0 57.8 58.0 276 bp 1.0 79.4
infA-rps8_p2 CCGTTGGGAAGTGATTCAGT GGCGGAATGGGGATTGTAAT 50.0 50.0 57.8 58.0 275 bp 1.0 79.4
infA-rps8_p3 CCGTTGGGAAGTGATTCAGT TTTAGGCGGAATGGGGATTG 50.0 50.0 57.8 57.9 279 bp 1.0 78.9
infA-rps8_p4 ACCGTTGGGAAGTGATTCAG TTTAGGCGGAATGGGGATTG 50.0 50.0 57.8 57.9 280 bp 1.0 78.8
infA-rps8_p5 CCGTTGGGAAGTGATTCAGT TAGGCGGAATGGGGATTGTA 50.0 50.0 57.8 58.2 277 bp 1.0 77.9