Hotspot

psbH-petB

LSC · 71146-71778 · 633 bp

Pi 0.00527

MarkerSeek score 62.30

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 5
pi: 0.00527
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TGACCCAAAGATACGAAGCG	50.0	50.0	57.9	58.0	813-845 bp	1.0	79.7
psbH-petB_p2	GGAGTCGCAATGGCTCTATT	TGACCCAAAGATACGAAGCG	50.0	50.0	57.8	58.0	816-848 bp	1.0	78.6
psbH-petB_p3	TCGCAATGGCTCTATTTGCA	TGACCCAAAGATACGAAGCG	45.0	50.0	58.3	58.0	812-844 bp	1.0	78.5
psbH-petB_p4	ACTTATGGGAGTCGCAATGG	TGACCCAAAGATACGAAGCG	50.0	50.0	57.7	58.0	823-855 bp	1.0	77.9
psbH-petB_p5	CCACTTATGGGAGTCGCAAT	TGACCCAAAGATACGAAGCG	50.0	50.0	57.7	58.0	825-857 bp	1.0	77.9