Hotspot

clpP-psbB

LSC · 68072-68588 · 517 bp

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Pi 0.0103
MarkerSeek score 63.60
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
517
variable_sites
8
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CCAGCCCAACCAGAAACTAG 50.0 55.0 58.0 58.2 697-705 bp 1.0 79.5
clpP-psbB_p2 CGTCAACCCAAATTGCATCC CCAGCCCAACCAGAAACTAG 50.0 55.0 58.0 58.2 688-696 bp 1.0 79.5
clpP-psbB_p3 CGCACTATACGTCAACCCAA TGGACAGAAAGCAATCGACC 50.0 50.0 58.0 58.2 661-669 bp 1.0 79.4
clpP-psbB_p4 CGTCAACCCAAATTGCATCC TGGACAGAAAGCAATCGACC 50.0 50.0 58.0 58.2 652-660 bp 1.0 79.4
clpP-psbB_p5 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 613-621 bp 1.0 79.1