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rpl33-rps18

LSC · 63768-63926 · 159 bp

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Pi 0.0168
MarkerSeek score 63.20
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
159
variable_sites
4
pi
0.0168
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACACCCAATCG TATTCGATCCCCCGATCCAA 50.0 50.0 56.9 58.0 330-331 bp 1.0 72.1
rpl33-rps18_p2 CGACACAATACACCCAATCG TCTATTCGATCCCCCGATCC 50.0 55.0 56.9 58.2 332-333 bp 1.0 71.1
rpl33-rps18_p3 CGACACAATACACCCAATCG GGGGTAAACGCCTACGAAAA 50.0 50.0 56.9 58.2 307-308 bp 1.0 70.8
rpl33-rps18_p4 CGACACAATACACCCAATCGA TATTCGATCCCCCGATCCAA 47.6 50.0 58.4 58.0 330-331 bp 1.0 69.7
rpl33-rps18_p5 TCGACACAATACACCCAATCG TATTCGATCCCCCGATCCAA 47.6 50.0 58.4 58.0 331-332 bp 1.0 69.7