Hotspot

accD-psaI

LSC · 56018-56218 · 201 bp

Pi 0.0232

MarkerSeek score 67.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 7
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCTTATTCGACCCAATC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.4	57.9	375-638 bp	1.0	75.1
accD-psaI_p2	GACCCAATCGTACCACGTAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.3	57.9	364-627 bp	1.0	75.0
accD-psaI_p3	AGGGCTTATTCGACCCAATC	CCATTGCAATTGCCGGAAAA	50.0	45.0	57.4	58.2	369-632 bp	1.0	74.4
accD-psaI_p4	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAA	50.0	45.0	57.3	58.2	358-621 bp	1.0	74.3
accD-psaI_p5	GCTACATGGTTTCCTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.2	57.9	311-574 bp	1.0	73.9