Hotspot

atpB-rbcL

LSC · 51722-52440 · 719 bp

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Pi 0.00895
MarkerSeek score 65.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
719
variable_sites
9
pi
0.00895
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TGGGCCAATAATTTGAGCGA TCCAACGCTTGCTTTAGTCT 45.0 45.0 57.9 57.5 785-848 bp 1.0 79.4
atpB-rbcL_p2 CGATACGCCCTAGGTTTTGT TCCAACGCTTGCTTTAGTCT 50.0 45.0 57.7 57.5 768-831 bp 1.0 78.1
atpB-rbcL_p3 TGGGCCAATAATTTGAGCGA ACGCTTGCTTTAGTCTCTGT 45.0 45.0 57.9 57.2 781-844 bp 1.0 77.2
atpB-rbcL_p4 TGGGCCAATAATTTGAGCGA CCAACGCTTGCTTTAGTCTC 45.0 50.0 57.9 57.1 784-847 bp 1.0 76.7
atpB-rbcL_p5 CTGGGCCAATAATTTGAGCG TCCAACGCTTGCTTTAGTCT 50.0 45.0 57.5 57.5 786-849 bp 1.0 76.5