Hotspot

ndhC-trnV-UAC

LSC · 47981-48717 · 737 bp

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Pi 0.0172
MarkerSeek score 59.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
737
variable_sites
19
pi
0.0172
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTTTCTGGCCCTTCCCTAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.1 58.1 887-903 bp 1.0 74.2
ndhC-trnV-UAC_p2 TTTCTGGCCCTTCCCTAATC CCGAGAAAGTCTACGGTTCG 50.0 55.0 56.9 58.1 885-901 bp 1.0 72.4
ndhC-trnV-UAC_p3 TTCTGGCCCTTCCCTAATCG CCGAGAAAGTCTACGGTTCG 55.0 55.0 59.2 58.1 884-900 bp 1.0 72.1
ndhC-trnV-UAC_p4 TCGGGGCTAAAATTCCAGAA CCGAGAAAGTCTACGGTTCG 45.0 55.0 56.8 58.1 867-883 bp 1.0 71.9
ndhC-trnV-UAC_p5 TTTTCTGGCCCTTCCCTAATC CCGAGAAAGTCTACGGTTCG 47.6 55.0 57.6 58.1 886-902 bp 1.0 70.3