Hotspot

trnF-GAA-ndhJ

LSC · 45728-46570 · 843 bp

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Pi 0.0119
MarkerSeek score 66.20
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
843
variable_sites
14
pi
0.0119
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GCTAGGATGATGCACGGAAA TATTTACGCTCCCAGTGTGC 50.0 50.0 58.0 58.0 969-997 bp 1.0 84.5
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA TATTTACGCTCCCAGTGTGC 50.0 50.0 58.1 58.0 900-935 bp 1.0 84.2
trnF-GAA-ndhJ_p3 GCTAGGATGATGCACGGAAA GGGACTCCATTGCTGTCATT 50.0 50.0 58.0 57.9 1010-1038 bp 1.0 83.4
trnF-GAA-ndhJ_p4 GCTAGGATGATGCACGGAAA TGTGCCTACGATGTAGGGAT 50.0 50.0 58.0 57.9 977 bp 0.333333 52.3
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA TGTGCCTACGATGTAGGGAT 50.0 50.0 58.1 57.9 908 bp 0.333333 52.3