Hotspot

psbA

LSC · 309-1370 · 1062 bp

Back to Ophiorrhiza

Pi 0.000628

MarkerSeek score 33.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCCTATACAATAAGACCGGA	ATTTGTGTGCTTGGGAGTCC	40.9	50.0	55.5	58.4	1144 bp	1.0	41.3
psbA_p2	AACTCCTATACAATAAGACCGGA	ATTTGTGTGCTTGGGAGTCC	39.1	50.0	56.3	58.4	1145 bp	1.0	40.1
psbA_p3	ACTCCTATACAATAAGACCGGAT	ATTTGTGTGCTTGGGAGTCC	39.1	50.0	56.1	58.4	1144 bp	1.0	40.1
psbA_p4	ACTCCTATACAATAAGACCGG	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	54.0	58.4	1144 bp	1.0	40.1
psbA_p5	ACTCCTATACAATAAGACCGGA	AGAATTTGTGTGCTTGGGAGT	40.9	42.9	55.5	58.1	1147 bp	1.0	40.1