Hotspot

ndhF

SSC · 109814-112069 · 2256 bp

Back to Ophiorrhiza

Pi 0.00922

MarkerSeek score 49.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 31
pi: 0.00922
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAATACCGTCCCCCATTCT	AGAGCCTGCTGTGTCATAGA	50.0	50.0	57.8	58.1	2757-2775 bp	0.666667	64.9
ndhF_p2	CAGAATACCGTCCCCCATTC	AGAGCCTGCTGTGTCATAGA	55.0	50.0	57.7	58.1	2758-2776 bp	0.666667	64.4
ndhF_p3	ACCGTCCCCCATTCTTACTA	AGAGCCTGCTGTGTCATAGA	50.0	50.0	57.4	58.1	2752-2770 bp	0.666667	62.0
ndhF_p4	TACCGTCCCCCATTCTTACT	AGAGCCTGCTGTGTCATAGA	50.0	50.0	57.4	58.1	2753-2771 bp	0.666667	62.0
ndhF_p5	AGAATACCGTCCCCCATTCT	GAGCCTGCTGTGTCATAGAA	50.0	50.0	57.8	57.3	2756-2774 bp	0.666667	60.6