Hotspot

infA

LSC · 80167-80325 · 159 bp

Back to Ophiorrhiza

Pi 0.0252

MarkerSeek score 67.50

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 159
variable_sites: 6
pi: 0.0252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CGAGAAATTATGCCTCCCCC	CTTCAAATGTGCTTTACCTGGA	55.0	40.9	58.1	56.9	234-235 bp	1.0	56.6
infA_p2	CGAGAAATTATGCCTCCCCC	TCTTCAAATGTGCTTTACCTGG	55.0	40.9	58.1	56.9	235-236 bp	1.0	56.6
infA_p3	GAGAAATTATGCCTCCCCCG	CTTCAAATGTGCTTTACCTGGA	55.0	40.9	58.1	56.9	233-234 bp	1.0	56.6
infA_p4	GAGAAATTATGCCTCCCCCG	TCTTCAAATGTGCTTTACCTGG	55.0	40.9	58.1	56.9	234-235 bp	1.0	56.6
infA_p5	GAGAAATTATGCCTCCCCCG	TCAAATGTGCTTTACCTGGA	55.0	40.0	58.1	54.9	231-232 bp	1.0	56.5