Hotspot

petA-psbJ

LSC · 63258-64260 · 1003 bp

Back to Ophiorrhiza

Pi 0.0349

MarkerSeek score 73.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1003
variable_sites: 49
pi: 0.0349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	998-1131 bp	1.0	83.5
petA-psbJ_p2	TGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1005-1138 bp	1.0	81.1
petA-psbJ_p3	GTGTCCAAGGCCTTTTGTTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.5	57.6	1006-1139 bp	1.0	80.6
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	931-1064 bp	1.0	78.1
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	997-1130 bp	1.0	77.6