Hotspot

psaA-ycf3

LSC · 42049-42857 · 809 bp

Back to Ophiorrhiza

Pi 0.0135

MarkerSeek score 59.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 16
pi: 0.0135
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	841-869 bp	1.0	78.9
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	845-873 bp	1.0	78.7
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.5	58.3	840-868 bp	1.0	77.9
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	GATCAAGCCGCTGAGTACTG	50.0	55.0	58.5	58.4	913-941 bp	1.0	76.4
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	CTGAGTACTGGAAACGTGCT	50.0	50.0	58.5	57.6	903-931 bp	1.0	76.4