Hotspot

trnT-GGU-psbD

LSC · 31868-33104 · 1237 bp

Back to Ophiorrhiza

Pi 0.00755

MarkerSeek score 64.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 14
pi: 0.00755
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1393-1404 bp	1.0	80.3
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1411-1422 bp	1.0	79.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1356-1367 bp	1.0	78.9
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CGGTCCCTACGTAACCAATC	50.0	55.0	57.9	57.8	1389-1400 bp	1.0	78.7
trnT-GGU-psbD_p5	TCCACCTAGTGACCCATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.7	58.0	1429-1440 bp	1.0	78.5