Hotspot

psbM-trnD-GUC

LSC · 29725-30608 · 884 bp

Back to Ophiorrhiza

Pi 0.0747

MarkerSeek score 89.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 98
pi: 0.0747
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	37.5	50.0	57.4	58.5	1002-1005 bp	1.0	50.0
psbM-trnD-GUC_p2	GGAACTAGAATGAATAGTGCAGT	TTCAATTGGTCAGAGCACCG	39.1	50.0	56.1	58.5	1001-1004 bp	1.0	50.0
psbM-trnD-GUC_p3	CGTAGGAACTAGAATGAATAGTGC	TTCAATTGGTCAGAGCACCG	41.7	50.0	57.0	58.5	1005-1008 bp	1.0	49.9
psbM-trnD-GUC_p4	AGGAACTAGAATGAATAGTGCAGT	CGCCCCTAGGTCCCTTATTT	37.5	55.0	57.4	58.9	1036-1039 bp	1.0	49.6
psbM-trnD-GUC_p5	TTGACTGACTGTTTTTACGT	TTCAATTGGTCAGAGCACCG	35.0	50.0	53.0	58.5	1044-1047 bp	1.0	49.6