Hotspot

petN-psbM

LSC · 28911-29619 · 709 bp

Back to Ophiorrhiza

Pi 0.00846

MarkerSeek score 65.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 9
pi: 0.00846
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.0	58.0	787-836 bp	1.0	74.6
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.0	58.0	785-834 bp	1.0	74.6
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.0	57.9	783-832 bp	1.0	74.1
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.0	57.9	781-830 bp	1.0	74.1
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACGGCCT	55.0	47.6	57.9	58.0	784-833 bp	1.0	74.0