Hotspot

trnS-GCU-trnG-GCC

LSC · 8436-8994 · 559 bp

Back to Ophiorrhiza

Pi 0.0203

MarkerSeek score 59.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 17
pi: 0.0203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	633-676 bp	1.0	53.5
trnS-GCU-trnG-GCC_p2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	682-725 bp	1.0	52.4
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	634-677 bp	1.0	51.6
trnS-GCU-trnG-GCC_p4	TGGAAACGGAAAGAGAGGGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.3	55.5	687-730 bp	1.0	51.4
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	683-726 bp	1.0	50.5