Hotspot

rps16-trnQ-UUG

LSC · 6151-7119 · 969 bp

Back to Ophiorrhiza

Pi 0.0217
MarkerSeek score 64.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
969
variable_sites
29
pi
0.0217
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1042-1679 bp 1.0 80.8
rps16-trnQ-UUG_p2 TCATTCCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1053-1690 bp 1.0 80.4
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1046-1683 bp 1.0 76.1
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1072-1709 bp 1.0 76.0
rps16-trnQ-UUG_p5 TCATTCCCTTCAAGTCGCAC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.2 57.3 1057-1694 bp 1.0 75.7