Hotspot

ycf1

SSC · 125893-131421 · 5529 bp

Back to Ophiopogon

Pi 0.00687

MarkerSeek score 47.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5529
variable_sites: 87
pi: 0.00687
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTACGTCTTTGAGCACGCAT	TTCAAGAAAAGGCAAACGCG	45.0	45.0	58.2	58.2	207-267 bp	1.0	83.3
ycf1_p2	TTATACCCCGGCGAAAATCC	TTCAAGAAAAGGCAAACGCG	50.0	45.0	57.7	58.2	176-236 bp	1.0	83.2
ycf1_p3	TTTACGTCTTTGAGCACGCA	TTCAAGAAAAGGCAAACGCG	45.0	45.0	58.4	58.2	208-268 bp	1.0	81.6
ycf1_p4	CCTTTGATTATACCCCGGCG	TTCAAGAAAAGGCAAACGCG	55.0	45.0	58.5	58.2	183-243 bp	1.0	81.4
ycf1_p5	CTTTGATTATACCCCGGCGA	TTCAAGAAAAGGCAAACGCG	50.0	45.0	57.5	58.2	182-242 bp	1.0	80.9