Hotspot

rps3

LSC · 84276-84938 · 663 bp

Back to Ophiopogon

Pi 0.00452

MarkerSeek score 48.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 7
pi: 0.00452
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGAACTCTGCAATGGAGCTC	CGGGCGTTCTTATGAGGAAA	50.0	50.0	57.9	58.0	1661-1699 bp	1.0	81.7
rps3_p2	TGAACTCTGCAATGGAGCTC	TTATCATTCATTGGCGGGGG	50.0	50.0	57.9	58.0	1789-1827 bp	1.0	81.6
rps3_p3	CTGAACTCTGCAATGGAGCT	TTATCATTCATTGGCGGGGG	50.0	50.0	57.9	58.0	1790-1828 bp	1.0	81.6
rps3_p4	TGAACTCTGCAATGGAGCTC	TCTGACTTTCGCGAGACATG	50.0	50.0	57.9	58.0	1861-1899 bp	1.0	81.5
rps3_p5	CTGAACTCTGCAATGGAGCT	TCTGACTTTCGCGAGACATG	50.0	50.0	57.9	58.0	1862-1900 bp	1.0	81.4