Hotspot

ndhA

SSC · 121853-124008 · 2156 bp

Back to Ophiopogon

Pi 0.00606

MarkerSeek score 62.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2156
variable_sites: 31
pi: 0.00606
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGAACATATTGTGGATCTCCGT	ATTGGCCGATATCATGACGA	45.5	45.0	57.9	56.9	2334-2353 bp	1.0	55.8
ndhA_p2	GGAACATATTGTGGATCTCCGT	TTGGCCGATATCATGACGAT	45.5	45.0	57.9	56.9	2333-2352 bp	1.0	55.8
ndhA_p3	GGAACATATTGTGGATCTCCGT	AATTGGCCGATATCATGACGA	45.5	42.9	57.9	57.6	2335-2354 bp	1.0	53.6
ndhA_p4	GGAACATATTGTGGATCTCCGT	TGGCCGATATCATGACGATAC	45.5	47.6	57.9	57.5	2332-2351 bp	1.0	52.8
ndhA_p5	GGAACATATTGTGGATCTCCGT	TGGGAGAAGTTGATCGTTGA	45.5	45.0	57.9	56.5	2290-2309 bp	1.0	52.8