Hotspot

ccsA

SSC · 116337-117308 · 972 bp

Back to Ophiopogon

Pi 0.00556

MarkerSeek score 48.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 12
pi: 0.00556
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1483-1489 bp	1.0	81.5
ccsA_p2	TGAAATCGGTAGACACGCTG	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1482-1488 bp	1.0	81.5
ccsA_p3	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1707-1713 bp	1.0	81.4
ccsA_p4	GAGTGGCGGCATACTGTAAA	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1423-1429 bp	1.0	81.4
ccsA_p5	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1706-1712 bp	1.0	81.3